Thermo Fisher Scientific TPH1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:500

View 1 publication 1 publication

Immunoprecipitation (IP)

1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rabbit, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to the C-terminal region of TPH1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TPH1,uniProtId:P17752-1,ncbiNodeId:9606,antigenRange:444,antigenLength:444,antigenImageFileName:PA1-777_TPH1_P17752-1_Rabbit.svg,antigenImageFileNamePDP:PA1-777_TPH1_P17752-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2256401

Product Specific Information

PA1-777 detects TPH1 in human, mouse, rat, rabbit and non-human primate samples.

PA1-777 has been successfully used in ICC/IF, IP, and Western blot procedures. By Western blot PA1-777 detects a 51 kDa protein representing TPH1.

The PA1-777 immunogen is a synthetic peptide corresponding to the C-terminal region of TPH1.

Target Information

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CAM K II) (Jiang et al., 2000; Johansen et al., 1996). Both PKA and CAM K II phosphorylate Ser58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.