Thermo Fisher Scientific nNOS Polyclonal Antibody
Rabbit polyclonal antibody against human nNOS. Validated for WB and IHC-P with 1:1,000 dilution. Recognizes human, mouse, and rat. Lyophilized form, reconstitute in sterile water. Suitable for neuroscience and nitric oxide synthase studies.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:1,000 | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from human nNOS conjugated to blue carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 150 µL of sterile water.
- Centrifuge to remove any insoluble material.
- The peptide is homologous in mouse and shares 96% identity with rat sequence.
- Specificity: nNOS.
Target Information
Nitric oxide synthase (NOS) activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH, and O₂) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme).
Several distinct NOS isoforms have been described, representing products of three distinct genes:
- NOS1 (neuronal, nNOS)
- NOS2 (inducible, iNOS)
- NOS3 (endothelial, eNOS)
NOS1 activity was first identified in neurons, and NOS3 in endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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