Thermo Fisher Scientific NGF Polyclonal Antibody
Rabbit polyclonal antibody against NGF for WB and IHC(P) applications. Reacts with human, mouse, and rat. Lyophilized form, reconstitute with sterile water. Store at 4°C short term or -20°C long term. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 | – |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 | – |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
Product Specifications
| Category | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from amino acid region 130–180 of mouse NGF conjugated to a carrier protein was used as the antigen. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at −20°C and avoid freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 100 µL of sterile water.
- Centrifuge to remove any insoluble material.
- The peptide is homologous in rat and human.
- Specificity: NGF.
Target Information
Nerve growth factor (NGF) influences the survival and differentiation of various neuronal and non-neuronal cells. Its effects result from binding to specific cell surface receptors with both high and low affinity sites. Only the high-affinity receptor is capable of signal transduction. Cross-linking studies with ¹²⁵I-NGF have shown that the high-affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF, while the low-affinity receptor forms an Mr 100,000 complex.
Full-length cDNA clones for both human and rat NGF receptor genes show over 90% homology between species. NGF regulates growth and differentiation of sympathetic and certain sensory neurons, and mutations in the NGF gene are associated with hereditary sensory and autonomic neuropathy type 5 (HSAN5). Dysregulation of NGF expression is linked to allergic rhinitis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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