Thermo Fisher Scientific Phospho-CtIP (Ser327) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:,1000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser327 of human CtIP, conjugated to keyhole limpet hemocyanin (KLH).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

10mM HEPES, pH 7.5, with 100µg/mL BSA, 50% glycerol, 150mM NaCl

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942855

Product Specific Information

Specific for endogenous levels of the ~100 kDa CtIP protein phosphorylated at Ser327. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Target Information

CtIP, a 125 kD protein, was originally found interacting with a transcription repressor, CtBP, through the PLDLS motif (CtBP-Interacting Protein) thus suggested a role in transcription. Studies have shown that CtIP also interacts with BRCA1 protein through the c-terminus BRCT domains also suggested that CtIP is a potential tumor suppressor. This CtIP-BRCA1 interaction can be disrupted by DNA damaging agents including UV or gamma-irradiation. Li et al (Nature 406, 210 - 215 (2000)) have shown that ATM phosphorylates CtIP at serine residues 664 and 745, and mutation of these sites abrogates the dissociation of BRCA1 from CtIP, resulting in persistent repression of BRCA1-dependent induction of GADD45 upon ionizing radiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.