Thermo Fisher Scientific Phospho-IRE1 alpha (Ser724) Polyclonal Antibody
Phospho-IRE1 alpha (Ser724) 폴리클로날 항체로 마우스 IRE1 단백질의 Ser724 인산화를 특이적으로 검출. Western blot에 적합하며 항원 친화 크로마토그래피로 정제됨. 연구용으로만 사용 가능.
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Applications
- Western Blot (WB): 1:1,000–1:5,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide corresponding to amino acid residues surrounding phospho Ser724 of mouse IRE1, conjugated to keyhole limpet hemocyanin (KLH) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | ~0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | 10 mM HEPES, pH 7.5, with 100 µg/mL BSA, 50% glycerol, 150 mM NaCl |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2942856 |
Product Specific Information
Specific for endogenous levels of the ~130 kDa GluR1 protein phosphorylated at Ser724. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
Target Information
ERN1 (endoplasmic reticulum to nucleus signaling 1) is a serine/threonine kinase and endoribonuclease that mediates ER stress signaling. It is a human homologue of the yeast Ire1 gene product and plays a critical role in ER stress response by splicing XBP1 mRNA to activate unfolded protein response transcription. IRE1 is essential for cell viability under stress conditions that cause unfolded protein accumulation in the ER. Null mutants are viable under normal conditions but undergo apoptosis under ER stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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