Thermo Fisher Scientific Ki-67 Polyclonal Antibody
Ki-67 단백질을 인식하는 다클론 항체로, 세포 증식 마커 연구에 적합합니다. Western blot, IHC, ICC 등 다양한 응용에 사용 가능하며, Human, Mouse, Rat 시료 반응성이 있습니다. 고농도 액상 형태로 제공되며, 장기 보관 시 -20°C에서 안정적으로 유지됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:1,000–1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Chicken / IgY |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant human Ki-67 protein expressed in and purified from E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | ~20.0 mg/mL |
| Purification | IgY fraction |
| Storage Buffer | PBS |
| Contains | 10 mM sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2942857 |
Product Specific Information
This antibody is specific for endogenous levels of the ~345 and 395 kDa Ki-67 isoforms. Additional bands are thought to be breakdown products of these two isoforms.
Target Information
Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly during late G1, S, G2, and M phases. It contains a forkhead-associated (FHA) domain that mediates association with euchromatin at the perichromosomal layer, centromeric heterochromatin, and the nucleolus.
Ki-67 shows a cell cycle-dependent localization pattern:
- G1 phase: Perinucleolar expression
- G2 phase: Expression in the nuclear matrix
- M phase: Expression on chromosomes
As Ki-67 is absent in G0 cells and increases steadily from G1 through mitosis, it serves as a reliable marker of cell proliferation. Ki-67 antibodies are widely used to determine the growth fraction in neoplasms, with strong correlation between low Ki-67 index and histologically low-grade tumors. It is also routinely used as a neuronal marker for cell cycling and proliferation studies.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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