Thermo Fisher Scientific MECP2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:250

-

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

Full length human recombinant MeCP2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

20 mg/mL

Purification

IgY fraction

Storage buffer

PBS

Contains

5mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2942858

Product Specific Information

Specific for endogenous levels of the MeCP2 protein. Due to usually high charge, the protein runs at ~75 kDa in SDS-PAGE of mouse brain lysates.

Target Information

MECP2 belongs to a family of nuclear proteins (including MBD1, MBD2, MBD3 and MBD4) that have a methyl-CpG binding domain. MECP2 is capable of binding specifically to methylated DNA. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. MECP2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.