Thermo Fisher Scientific Fibronectin Monoclonal Antibody (FN1, 3029)
Thermo Fisher Scientific의 Fibronectin Monoclonal Antibody (FN1, 3029)는 인간 Fibronectin 단백질을 인식하는 Mouse IgG 항체입니다. IHC 및 ELISA에 적합하며, 고순도의 Protein A/G 정제 형태로 제공됩니다. 세포 부착 및 이동 연구에 활용 가능하며, 연구용으로만 사용됩니다.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–2 µg/mL
Peptide Array (Array)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | FN1, 3029 |
| Immunogen | Recombinant fragment (around aa 467–595) of human fibronectin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Antibody is tested in direct ELISA.
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Fibronectin is a disulfide-bonded dimer (230–250 kDa) found in the extracellular matrix of connective tissues and vessels. It exists as an insoluble, cross-linked protein forming high molecular mass polymers.
Fibronectin plays key roles in:
- Embryonic development and tissue remodeling
- Cell adhesion and migration
- Wound healing, blood coagulation, host defense, and metastasis
Fibronectin expression increases in non-small cell lung carcinoma, enhancing tumorigenicity and resistance to apoptosis. The fibronectin gene undergoes alternative splicing, potentially producing up to 20 transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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