Thermo Fisher Scientific PAR2 Monoclonal Antibody (SAM11), eBioscience
인간 PAR2 단백질을 인식하는 단클론 항체로, 다양한 염증 질환 및 암 연구에 활용 가능. WB, IHC, ICC, ELISA, 기능 분석 등 다양한 응용에 적합. 고순도(>90%)로 정제된 액상 항체이며 4°C에서 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:250 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 10 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | Assay-Dependent |
| Functional Assay | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SAM11 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572959 |
Product Specific Information
The monoclonal antibody SAM11 recognizes human PAR2 (Protease Activated Receptor 2), a member of the PAR family within the G protein-coupled receptor (GPCR) group. Unlike most GPCRs, PARs are activated through proteolytic cleavage rather than ligand binding. PAR2 activation is induced by enzymes such as trypsin, mast cell tryptase, neutrophil proteinase 3, tissue factor-factor VIIa-factor Xa, kallikrein-related peptidases, and matriptase-1. It is not activated by thrombin.
PAR2 is widely expressed in tissues including skin, liver, kidney, small intestine, and colon, and plays a key role in inflammatory diseases and cancer. The SAM11 antibody has been reported to inhibit serine proteinase-mediated activation of PAR2.
Applications Reported:
This antibody can be used for immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections, microscopy, ELISA, functional assays, and immunocytochemistry.
Applications Tested:
- Tested in IHC (P) using low or high pH antigen retrieval.
- Tested in ICC/IF with methanol or formaldehyde-fixed and permeabilized cells.
- Recommended concentration ≤10 µg/mL.
- Optimal dilution should be determined experimentally.
Purity: Greater than 90% (SDS-PAGE)
Aggregation: Less than 10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Coagulation factor II (thrombin) receptor-like 1 (F2RL1) is a 7-transmembrane receptor coupled to G proteins and part of the protease-activated receptor family. It is activated by trypsin but not thrombin, via proteolytic cleavage of its extracellular amino terminus, generating a tethered ligand that activates the receptor. The F2RL1 gene has two exons and is widely expressed in human tissues. The predicted protein sequence shares 83% identity with the mouse receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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