Thermo Fisher Scientific CD366 (TIM3) Monoclonal Antibody (8B.2C12), Alexa Fluor 488, eBioscience

Thermo Fisher Scientific CD366 (TIM3) Monoclonal Antibody (8B.2C12), Alexa Fluor 488

Applications & Tested Dilution

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• Functional Grade
• PE
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Custom conjugation available

Product Specific Information

The 8B.2C12 monoclonal antibody reacts with mouse CD366 (TIM3), a Th1-specific cell surface protein. CD366 is a type I transmembrane protein containing an immunoglobulin and mucin-like domain extracellularly, and a tyrosine phosphorylation motif in its cytoplasmic portion.
It is selectively expressed by differentiated CD4+ Th1 and CD8+ Tc1 cells, but absent on Th2, Tc2, and other hematopoietic cells such as naïve T cells, B cells, macrophages, and dendritic cells.

Expression of CD366 increases at the late stage of Th1 differentiation, suggesting a role in effector function rather than differentiation. In EAE models, CD366 is expressed on most CD4+ and CD8+ T cells in the CNS at disease onset, while peripheral expression remains low. Administration of 8B.2C12 antibody induces a hyperacute disease phenotype, implicating CD366 in macrophage activation and autoimmune severity.

The 8B.2C12 antibody binds strongly to the BALB/c allele of CD366, but weakly to the C57Bl/6 allele.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse splenocytes (≤0.5 µg/test, 100 µL final volume)
Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser

Target Information

TIM3 (Hepatitis A virus cellular receptor 2, HAVCR2, T-cell immunoglobulin and mucin-domain containing-3) is a 281 amino acid, type I Th1-specific cell surface glycoprotein expressed on terminally differentiated CD4+ Th1 and CD8+ Tc1 cells.
It includes an IgV-like domain, a mucin-like domain, and a conserved tyrosine phosphorylation motif. TIM3 regulates macrophage activation, autoimmunity, and immune tolerance.
It has pathological significance in Th1-dependent autoimmune diseases such as experimental autoimmune encephalomyelitis (EAE).

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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