Thermo Fisher Scientific CD366 (TIM3) Monoclonal Antibody (8B.2C12), Alexa Fluor 488, eBioscience
마우스 CD366(TIM3)을 인식하는 8B.2C12 클론의 Rat IgG1 단클론 항체로, Alexa Fluor 488 형광 표지. Flow Cytometry에 최적화되어 있으며, Th1/Tc1 세포 표면 단백질 분석에 유용. 499/520 nm 형광 특성, 4°C 보관, 연구용 전용.
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Thermo Fisher Scientific CD366 (TIM3) Monoclonal Antibody (8B.2C12), Alexa Fluor 488
Applications & Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | 4 publications |
| Immunoprecipitation (IP) | - | 1 publication |
| Neutralization (Neu) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host/Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Alexa Fluor™ 488 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8B.2C12 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation/Emission Max | 499/520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2848436 |
Additional Formats Available
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- Functional Grade
- PE
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Custom conjugation available
Product Specific Information
The 8B.2C12 monoclonal antibody reacts with mouse CD366 (TIM3), a Th1-specific cell surface protein. CD366 is a type I transmembrane protein containing an immunoglobulin and mucin-like domain extracellularly, and a tyrosine phosphorylation motif in its cytoplasmic portion.
It is selectively expressed by differentiated CD4+ Th1 and CD8+ Tc1 cells, but absent on Th2, Tc2, and other hematopoietic cells such as naïve T cells, B cells, macrophages, and dendritic cells.
Expression of CD366 increases at the late stage of Th1 differentiation, suggesting a role in effector function rather than differentiation. In EAE models, CD366 is expressed on most CD4+ and CD8+ T cells in the CNS at disease onset, while peripheral expression remains low. Administration of 8B.2C12 antibody induces a hyperacute disease phenotype, implicating CD366 in macrophage activation and autoimmune severity.
The 8B.2C12 antibody binds strongly to the BALB/c allele of CD366, but weakly to the C57Bl/6 allele.
Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse splenocytes (≤0.5 µg/test, 100 µL final volume)
Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser
Target Information
TIM3 (Hepatitis A virus cellular receptor 2, HAVCR2, T-cell immunoglobulin and mucin-domain containing-3) is a 281 amino acid, type I Th1-specific cell surface glycoprotein expressed on terminally differentiated CD4+ Th1 and CD8+ Tc1 cells.
It includes an IgV-like domain, a mucin-like domain, and a conserved tyrosine phosphorylation motif. TIM3 regulates macrophage activation, autoimmunity, and immune tolerance.
It has pathological significance in Th1-dependent autoimmune diseases such as experimental autoimmune encephalomyelitis (EAE).
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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