Thermo Fisher Scientific COX2 Polyclonal Antibody

Thermo Fisher Scientific COX2 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody is predicted to react with bovine, mouse and rabbit based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is colon, lung or breast carcinoma.

Target Information

COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis, including production of inflammatory prostaglandins.
The conversion of arachidonate to prostaglandin H2 is a two-step reaction: a cyclooxygenase (COX) reaction which converts arachidonate to prostaglandin G2 (PGG2), and a peroxidase reaction in which PGG2 is reduced to prostaglandin H2 (PGH2).

COX2 is constitutively expressed in some tissues under physiological conditions (endothelium, kidney, brain) and up-regulated under pathological conditions such as cancer and inflammation.
Up-regulation of COX2 is associated with increased cell adhesion, phenotypic changes, resistance to apoptosis, and tumor angiogenesis.
In cancer cells, COX2 plays a key role in producing prostaglandin E2 (PGE2), which modulates motility, proliferation, and resistance to apoptosis.
COX2 is naturally inhibited by calcitriol (active form of Vitamin D).
Glucocorticoids repress PTGS2 gene activity in vivo by interfering with transcription initiation and elongation.
COX2 is a target of NSAIDs such as aspirin, which reduce pain and swelling from COX2-driven inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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