Thermo Fisher Scientific Claudin 7 Polyclonal Antibody
Claudin 7 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, Western blot 및 IHC(파라핀 포함)에 사용 가능. 인간, 생쥐, 랫드 반응성. 항원 친화 크로마토그래피로 정제되었으며, PBS 버퍼에 1% BSA 포함. 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:100–1:1,000 | - |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide from C-terminus of human claudin 7 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.12 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2276504 |
Product Specific Information
This antibody is predicted to react with bovine, canine, mouse, porcine, and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is colon carcinoma.
Target Information
This gene encodes a member of the claudin family, integral membrane proteins that are components of tight junction strands.
Tight junctions act as barriers regulating solute and water passage through paracellular spaces between epithelial or endothelial cells, maintaining cell polarity and signal transduction.
Differential expression of this gene has been observed in various malignancies, including breast, ovarian, hepatic, urinary, prostate, lung, head and neck, and thyroid cancers.
Alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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