Thermo Fisher Scientific SRPK2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human SRPK2. Recombinant protein control fragment (Product #RP-91439). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SRPK2,uniProtId:P78362-1,ncbiNodeId:9606,antigenRange:396-516,antigenLength:688,antigenImageFileName:PA5-53315_SRPK2_P78362-1_Rabbit.svg,antigenImageFileNamePDP:PA5-53315_SRPK2_P78362-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.10 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2647929

Product Specific Information

Immunogen sequence: QQLDDEDDDE EDCPNPEEYN LDEPNAESDY TYSSSYEQFN GELPNGRHKI PESQFPEFST SLFSGSLEPV ACGSVLSEGS PLTEQEESSP SHDRSRTVSA SSTGDLPKAK TRAADLLVNP L

Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 83%.

Target Information

SRPK2, along with SRPK1, is most likely the cellular protein kinase mediating HBV core protein phosphorylation during viral infection. It therefore represents an important host cell target for therapeutic intervention in HBV infection. Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression. This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression. Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up-regulation. Plays an essential role in spliceosomal B complex formation via the phosphorylation of DDX23/PRP28. Plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles .

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.