Thermo Fisher Scientific TYW1 Polyclonal Antibody
Human TYW1 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB 및 IHC(P) 실험에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS 기반 버퍼에 보존됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human TYW1. Recombinant protein control fragment (Product # RP-90614) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.05 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2649152 |
Product Specific Information
Immunogen sequence:
RRAMITPALR EALTKQGYQL IGSHSGVKLC RWTKSMLRGR GGCYKHTFYG IESHRCMETT PSLACANKCV FCWRHHTNPV GTEWRWKMDQ PEMILKEAIE NHQNMIKQFK GVPGVKA
Antigen sequence identity:
- Mouse: 97%
- Rat: 96%
Target Information
TYW1 (also known as TYW1A, RSAFD1, or YPL207W) is a 732 amino acid protein containing one flavodoxin-like domain that participates in the wybutosine-tRNA (Phe) biosynthesis pathway.
Wybutosine (yW) is a hypermodified guanosine at the 3′ position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome.
TYW1 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during ribosomal decoding, ensuring correct translation.
It binds one 4Fe-4S cluster and is located on human chromosome 7.
Defects in genes on chromosome 7 have been linked to Osteogenesis imperfecta, Williams-Beuren syndrome, Lissencephaly, Citrullinemia, and Shwachman-Diamond syndrome, suggesting that TYW1 may play a role in these conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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