Thermo Fisher Scientific CD27 Monoclonal Antibody (LG.7F9), Functional Grade, eBioscience

Thermo Fisher Scientific CD27 Monoclonal Antibody (LG.7F9), Functional Grade, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661
• Brilliant Violet 650
• Brilliant Violet 786
• FITC
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46-CD8- precursors.
CD27 binds to CD70 and plays an important role in T cell-B cell interaction and effector cell maturation. LG.7F9 cross-reacts with human and rat CD27.

Applications Reported: Flow cytometric analysis and ligand blocking.
Applications Tested: Tested on mouse splenocytes; ≤0.5 µg/test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Optimize titration for best performance.

Storage and Handling: Use in sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10% (HPLC).

Target Information

CD27 is a 50 kDa member of the TNF receptor superfamily (including CD40 and CD30), involved in regulating cell proliferation and death.
Expression of CD27 and its ligand CD70 is mainly confined to lymphocytes. CD27 is a disulfide-linked homodimer found on mature thymocytes, peripheral T cells, and some B cells.
Activation via TCR-CD3 complex increases CD27 expression and releases soluble CD27 (28–32 kDa), a prognostic marker for B cell malignancies.
CD27-binding protein (SIVA) is a proapoptotic factor that mediates apoptosis through CD27.
Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.