Thermo Fisher Scientific PTGER3 Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) [IHC (P)]

• Tested Dilution: 4 µg/mL
• 참고: Heat induced antigen retrieval in pH 6.0 citrate buffer 권장

ELISA

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• Percent identity with other species by BLAST analysis:
  Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Bat, Panda, Horse, Rabbit (100%);
  Elephant, Pig (94%); Monkey, Bovine (89%)
• For IHC(P): Use heat induced antigen retrieval in pH 6.0 citrate buffer.
  After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Target Information

CD172a, also known as signal-regulatory protein alpha, is a receptor-type transmembrane glycoprotein expressed on myeloid-origin cells such as granulocytes, dendritic cells (DCs), macrophages, mast cells, and hematopoietic stem cells.
It acts as a substrate for several activated tyrosine kinases (EGFR, PDGFR, src, insulin receptor) and participates in the negative regulation of receptor tyrosine kinase-coupled signaling pathways.
Ligand binding of CD172a to CD47 induces phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs), recruiting SHP-1 and SHP-2 phosphatases that regulate cellular functions through substrate dephosphorylation.
The CD172a-CD47 interaction is involved in processes such as inhibition of macrophage phagocytosis and bi-directional activation of T cells and DCs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.