Thermo Fisher Scientific Human SIK3, GST Tag Recombinant Protein

Applications

• Kinase Assay (KA)

Tested Dilution: Assay-dependent
Publications: Refer to Thermo Fisher resources.

Product Specifications

Product Specific Information

• Spin briefly prior to use for maximum recovery.
• Aliquots of 5 µg, 10 µg, and 20 µg sizes are not recommended; use material directly from original packaging.
• For larger sizes, aliquoting may reduce freeze/thaw cycles; avoid aliquots below 20 µL.
• Do not store kinase diluted.
• Guaranteed for 6 months from date of purchase if stored at -80°C.
• Recombinant human SIK3 (1–307) expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Key Features:

• Validated in LanthaScreen™ Kinase Binding Assay
• Protocols available for optimized performance
• Multiple pack sizes for convenience

Related Product:
LanthaScreen™ Eu Kinase Binding Assay

Protein Form: Recombinant

Target Information

SIK3 (QSK) is a serine/threonine-protein kinase belonging to the QIK subfamily. Phosphorylation by LKB1 via 14-3-3 binding enhances catalytic activity and localizes SIK3 to punctate cytoplasmic structures. Overexpression promotes G1/S cell cycle progression, notably in ovarian cancer. Mutations (H331L, A1103V) occur frequently in breast cancer. SIK3 acts as a tumor-associated antigen and positive regulator of mTOR signaling through DEPTOR degradation. It plays a role in mTOR signaling regulation during chondrocyte differentiation and negatively regulates cAMP signaling via CRTC2/TORC2 and CRTC3/TORC3.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.