Thermo Fisher Scientific HSF1 Monoclonal Antibody (4B4), APC
HSF1 단백질을 인식하는 Rat monoclonal 항체로, APC 형광 표지. Western blot, IHC, ICC, ELISA, IP, Gel Shift 등 다양한 응용 가능. Human, Mouse 등 여러 종에 반응. 단백질 G 정제, 4°C 보관. 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
| Gel Shift (GS) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Guinea pig, Hamster, Human, Mouse, Non-human primate, Rabbit, Rat |
| Host / Isotype | Rat / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4B4 |
| Immunogen | Purified recombinant mouse HSF1 protein (epitope mapping to amino acids 425–439) |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932166 |
Additional Formats
- FITC (Cat. No. MA5-45713)
- PerCP (Cat. No. MA5-45714)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45712 was sufficient for detection of HSF1 in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot using Rabbit anti-rat IgG: AP as the secondary antibody. Detects approximately 85 kDa (unstressed cell lysates) and approximately 95 kDa (heat-shocked cell lysates).
Target Information
All organisms respond to elevated temperatures and various environmental stresses by rapid synthesis of heat shock RNAs and proteins. The transcriptional activator, heat shock factor (HSF), mediates regulation of heat shock gene transcription by binding to heat shock response elements (HSEs), which consist of three repeats of a 5-nucleotide {nGAAn} module. These sequences are conserved across species.
Two HSFs have been identified in human cells: HSF1 and HSF2. HSF1 responds to classical stress signals such as heat, heavy metals, and oxidative reagents, while HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and nucleus as a monomer lacking DNA-binding activity. Upon stress, HSF1 becomes activated to a nuclear-localized trimeric form that binds DNA. Phosphorylation of HSF1 is required for maximal transcription of heat shock genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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