Thermo Fisher Scientific Phospho-ERK1 (Thr202, Tyr204) Monoclonal Antibody (M206)
EGF 자극 시 ERK1/2 인산화 형태를 검출하는 단일클론 항체. Western blot과 ICC/IF에 적합하며, 인간·마우스·랫트 반응성. 단백질 A 정제, 액상 형태로 안정적이며 -20°C 보관. 세포 신호전달 연구에 활용 가능.
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Thermo Fisher Scientific Phospho-ERK1 (Thr202, Tyr204) Monoclonal Antibody (M206)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M206 |
| Immunogen | Dual phosphorylated ERK1 (Thr-202/Tyr-204) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Thr-202 and Tyr-204 in human ERK1. This sequence is conserved in rat and mouse ERK1, and highly conserved in ERK2. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942622 |
Product Specific Information
The antibody detects 42 and 44 kDa proteins corresponding to ERK1 and ERK2 on SDS-PAGE immunoblots of human A431 epithelial cells stimulated with EGF or calyculin A. It does not detect bands in control cells.
Target Information
MAPK3 (ERK1) is a serine/threonine kinase that activates p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. ERK1 is widely expressed and regulates meiosis, mitosis, and post-mitotic functions. It responds to various stimuli such as growth factors, cytokines, virus infection, and G protein-coupled receptor ligands. ERK1 participates in signaling cascades controlling proliferation, differentiation, and cell cycle progression. Activated ERK1 translocates to the nucleus to phosphorylate nuclear targets. Alternatively spliced transcript variants encoding different isoforms of ERK1 have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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