Thermo Fisher Scientific Phospho-JNK1 (Thr183, Tyr185) Monoclonal Antibody (M268)
인산화된 JNK1 (Thr183, Tyr185)을 특이적으로 인식하는 마우스 단클론 항체입니다. Western blot 및 ICC/IF에 적합하며, 인간·마우스·랫트 시료에서 반응합니다. 단백질 A로 정제된 액상 항체로, -20°C 보관 및 연구용으로 사용됩니다.
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Thermo Fisher Scientific Phospho-JNK1 (Thr183, Tyr185) Monoclonal Antibody (M268)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M268 |
| Immunogen | Dual phosphorylated peptide corresponding to amino acid residues surrounding Thr-183 and Tyr-185 in human JNK1. This sequence has high homology to conserved sites in rat and mouse JNK1 and JNK2. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942611 |
Product Specific Information
This antibody detects 46 and 54 kDa proteins corresponding to the apparent molecular mass of JNK1 and JNK2 on SDS-PAGE immunoblots of human A431 and HeLa cells, as well as rat PC12 cells treated with calyculin A.
Target Information
MAPK8 (JNK1) is activated by misfolded proteins in the endoplasmic reticulum and acts as a crucial mediator of insulin resistance and obesity. It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are serine-threonine protein kinases activated by various extracellular stimuli, mediating signal transduction from the cell surface to the nucleus.
JNK is involved in cellular processes such as proliferation, differentiation, transcription regulation, and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing, with JNK1 and JNK2 being predominant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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