Thermo Fisher Scientific IL-3 Polyclonal Antibody, Biotin
Biotin으로 결합된 Anti-Human IL-3 폴리클로날 항체로, 인간 IL-3 단백질을 인식합니다. Western blot, IHC, ELISA 등 다양한 면역분석에 적합하며, 고순도 IgG 형태로 제공됩니다. 연구용으로만 사용되며 장기 보관 시 -20°C 이하에서 안정적입니다.
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Thermo Fisher Scientific IL-3 Polyclonal Antibody, Biotin
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:20,000–1:100,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-Human IL-3 (Rabbit) Antibody Biotin Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full-length recombinant human IL-3 protein. |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid freeze/thaw cycles.
- Centrifuge product if not completely clear after standing at room temperature.
- Product is stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
- Biotin Conjugated Anti-Human IL-3 Antibody is an IgG fraction purified via delipidation, salt fractionation, and ion-exchange chromatography.
- Heated to 56°C for 30 minutes for stability.
- Recognizes both native and recombinant human IL-3 in cell supernatants and certain body fluids.
- A control of similarly diluted normal rabbit IgG is recommended.
Target Information
Interleukin 3 (IL-3), also known as Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells, and eosinophils.
IL-3 acts as a hematopoietic growth factor stimulating colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell, and monocytic lineages.
Its bioactivity is highly species-specific — human IL-3 has no activity on mouse cells.
IL-3 supports proliferation and differentiation of hematopoietic cell types and may also possess neurotrophic activity associated with neurologic disorders.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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