Thermo Fisher Scientific IL-32A Polyclonal Antibody, Biotin
IL-32A 단백질을 인식하는 비오틴 결합 폴리클로날 항체로, 인간 시료에 반응합니다. Western blot, IHC, ELISA 등 다양한 면역 분석에 적합합니다. 장기 보관 시 -20°C에서 안정하며, 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:20,000–1:100,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-32A protein. |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Storage Buffer | 0.02M potassium phosphate/whole serum, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-32A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Target Information
Interleukin-32 (IL-32) was initially identified as a transcript (NK4) selectively expressed in lymphocytes and NK cells, with expression increased following activation by IL-2. It was later re-isolated from an IL-18-treated lung carcinoma cell line and renamed IL-32.
IL-32 does not share sequence homology with known cytokine families and is highly expressed in immune tissues, existing in at least four differentially spliced isoforms.
Treatment of human monocytic and mouse macrophage cells with IL-32 induces several proinflammatory cytokines such as TNF-α, IL-8, and MIP-2. It is also induced in human peripheral lymphocyte cells after mitogen stimulation and in epithelial cells by IFN-γ.
These properties suggest that IL-32 may play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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