Thermo Fisher Scientific NAG-1 Polyclonal Antibody, Biotin
인간 및 마우스 NAG-1 단백질에 반응하는 Biotin 결합 폴리클로날 항체로, WB, IHC, ELISA, IP 등 다양한 응용에 적합합니다. Protein A 정제, Lyophilized 형태이며 안정적인 저장 조건을 제공합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific NAG-1 Polyclonal Antibody, Biotin
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:20,000–1:100,000 |
| Immunoprecipitation (IP) | 1:2,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Protein-A purified antibody prepared by repeated immunizations with an MBP-tagged recombinant protein corresponding to mature human NAG-1 protein. Cross-reactivity to MBP removed via cross-adsorption chromatography. |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This antibody reacts with endogenous NAG-1 protein from human tissues.
BLAST analysis suggests reactivity with NAG-1 from:
- Human (100% homology)
- Chimpanzee (98%)
- Macaque (94%)
- Mouse and rat (70%)
Cross-reactivity with NAG-1 from other species has not been determined.
Target Information
GDF-15 belongs to the TGF-beta cytokine family, which plays an important role in prenatal development, postnatal growth, and tissue remodeling.
It is mainly expressed in the placenta and at lower levels in other tissues.
Detected in amniotic fluid and elevated in sera of pregnant women, indicating involvement in gestation and embryonic development.
GDF-15 generally exerts tumor suppressive activities and is produced in response to activation of the p53 pathway.
Serum levels correlate with progression of certain tumor types, including colorectal, pancreatic, and prostate cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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