Thermo Fisher Scientific Cyclin B1 Recombinant Rabbit Monoclonal Antibody (RM281)
Cyclin B1 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF에 적합합니다. 높은 특이성과 재현성을 제공하며, HEK293 세포에서 발현된 IgG 형식의 항체입니다. 세포주기 G2/M 전이 연구 및 암 관련 단백질 분석에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM281 |
| Immunogen | A peptide corresponding to Cyclin B1 (UniProt ID: P14635-1, antigen length: 433 aa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744961 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
Cyclins regulate Cyclin-dependent kinases (CDKs). Cyclin B1 (CCNB1) is essential for controlling the cell cycle at the G2/M transition. It forms a complex with p34 (cdc2) to create the maturation-promoting factor (MPF).
Two alternative transcripts exist: one constitutively expressed and another cell cycle-regulated, predominantly during G2/M phase. Cyclin B1 accumulates during G2 phase and is degraded at the end of mitosis via APC-dependent mechanisms.
Cyclin B1 overexpression has been observed in various cancers, including breast, prostate, and non-small cell lung cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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