Thermo Fisher Scientific CD66e (CEA) Recombinant Rabbit Monoclonal Antibody (RM326)
Thermo Fisher Scientific의 CD66e (CEA) Recombinant Rabbit Monoclonal Antibody (RM326)은 인간 CEA 단백질을 타깃으로 하는 고특이성 항체입니다. Western blot, IHC, ICC, ELISA에 사용 가능하며, 재조합 기술로 생산되어 높은 재현성과 민감도를 제공합니다. 연구용으로 적합합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:2,000 |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| ELISA | 1:1,000–1:5,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM326 |
| Immunogen | Native CEA protein purified from human liver tissue |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744958 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select optimal clones for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the extensive rabbit immune repertoire
Target Information
CEA (Carcinoembryonic Antigen, CD66e) is expressed during fetal gut development and re-expressed in various carcinomas. It belongs to the immunoglobulin superfamily, containing one N domain and six immunoglobulin constant-like domains (A1, A2, A3, B1, B2, B3).
Antibodies against CEA are useful in:
- Identifying origins of metastatic adenocarcinomas
- Differentiating pulmonary adenocarcinomas (CEA+) from pleural mesotheliomas (rarely or weakly CEA+)
CEA functions as a cell adhesion molecule and is implicated in:
- Tissue differentiation and 3D structure organization
- Angiogenesis, apoptosis, tumor suppression, and metastasis
- Modulation of innate and adaptive immune responses
Multiple transcript variants exist, though not all full-length forms are defined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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