Thermo Fisher Scientific ID4 Monoclonal Antibody (OTI3H1), TrueMAB
ID4 단백질을 인식하는 Mouse monoclonal antibody (Clone OTI3H1). Western blot에 최적화되어 있으며, Human 시료에 반응합니다. Lyophilized 형태로 제공되며, PBS buffer와 trehalose로 안정화. 연구용으로만 사용 가능합니다.
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Applications
- Western Blot (WB): Tested dilution 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3H1 |
| Immunogen | Full length human recombinant protein of human ID4 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
ID4 is a transcription factor containing a basic helix-loop-helix (bHLH) motif that regulates expression of tissue-specific genes in mammalian and insect systems.
DNA-binding activity of bHLH proteins depends on homo- and/or heterodimer formation.
Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, contain the HLH-dimerization domain but lack the DNA-binding basic domain.
As a result, Id proteins inhibit DNA binding and transcriptional activation by heterodimerizing with bHLH proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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