Thermo Fisher Scientific ASK1 Monoclonal Antibody (2E4)
ASK1(MAP3K5)를 인식하는 Mouse monoclonal antibody(2E4)로, WB, IHC, ICC, Flow, ELISA에 사용 가능. Human 시료에 반응하며, 비결합형 액상 형태로 제공. 4°C 단기, -20°C 장기 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | - |
| Flow Cytometry (Flow) | 1:200–1:400 | - |
| ELISA | 1:10,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2E4 |
| Immunogen | Purified recombinant fragment of human MAP3K5 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11152150 |
Product Specific Information
MA5-15861 targets MAP3K5 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human samples.
The immunogen is a purified recombinant fragment of human MAP3K5 expressed in E. coli.
MA5-15861 detects MAP3K5, which has a predicted molecular weight of approximately 55 kDa.
Target Information
MAP3K5 (ASK1) is a serine/threonine kinase abundantly expressed in human heart and pancreas, activated by various extracellular stimuli such as cytokines, growth factors, and environmental stresses.
ASK1 (Apoptosis Signal-Regulating Kinase 1) activates two MAPKK subgroups, MKK4 and MKK6, which subsequently activate JNK and p38 MAP kinases.
ASK1/MAPKKK5 is activated by TNFR and Fas through interactions with TRAF family members and Fas-associated protein Daxx.
Overexpression of ASK1 induces apoptotic cell death, while a catalytically inactive form inhibits TNF-α–induced apoptosis. ASK1 is expressed in various human and mouse tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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