Thermo Fisher Scientific SMN1 Monoclonal Antibody (5H1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

Immunocytochemistry (ICC/IF)

1:200-1:1,000

-

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5H1

Immunogen

Purified recombinant fragment of human SMN1 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_11155034

Product Specific Information

MA5-15858 targets SMN1 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human and Non-human primate samples.

The MA5-15858 immunogen is purified recombinant fragment of human SMN1 expressed in E. Coli. .

MA5-15858 detects SMN1 which has a predicted molecular weight of approximately 39kDa.

Target Information

The SMN1 gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. The telomeric and centromeric copies of this gene are nearly identical and encode the same protein - survival motor neuron protein. The SMN complex plays a catalyst role in the assemble of small nuclear ribonucleoproteins, the building blocks of the spliceosome. Mutations in the SMN1 gene are known to cause spinal muscular atrophy 1/2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.