Thermo Fisher Scientific CD85d (ILT4) Monoclonal Antibody (42D1), Functional Grade, eBioscience
CD85d (ILT4) 단클론 항체(42D1)는 인간, 마우스, 비인간 영장류 시료에서 기능적 분석 및 유세포 분석에 적합합니다. ILT4 단백질을 인식하며 면역 반응 조절 연구에 활용됩니다. 고순도 정제, 낮은 엔도톡신 수준, 4°C 보관 안정성 제공.
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4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD85d (ILT4) Monoclonal Antibody (42D1), Functional Grade, eBioscience
Thermo Fisher Scientific CD85d (ILT4) Monoclonal Antibody (42D1), Functional Grade, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | View 4 publications |
| Functional Assay (Functional) | Assay-Dependent | View 1 publication |
Product Specifications
| Category | Specification |
|---|---|
| Published Species | Human, Mouse, Non-human primate |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 42D1 |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2866013 |
Available Formats
Product Specific Information
The monoclonal antibody 42D1 recognizes the CD85d protein (ILT4, LIR-2), a member of the ILT/LIR/MIR receptor family. CD85d is a 110 kDa single transmembrane receptor with three Ig-like domains and a cytoplasmic domain containing ITIMs. Expression is confined to monocytes, macrophages, and dendritic cells. Its ligand includes non-classical class I HLA-G, -B, and G1. Upon ligand binding, SHP-1 interacts with ITIMs, contributing to immune tolerance during fetal implantation and possibly organ allograft rejection.
Crosslinking of CD85d with the 42D1 antibody inhibits calcium flux and enhances HLA-G tetramer staining, restoring the stimulating activity of tolerogenic dendritic cells.
Applications Reported: Flow cytometric analysis and functional assays.
Applications Tested: Flow cytometric analysis of normal human peripheral blood cells. Recommended ≤1 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimal performance requires titration.
Storage and Handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10% (HPLC).
Target Information
The LILRB2 (ILT4) gene is part of the leukocyte immunoglobulin-like receptor (LIR) family located at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors with two to four extracellular Ig domains, a transmembrane region, and ITIM motifs. Expressed on immune cells, it binds MHC class I molecules on antigen-presenting cells to inhibit immune stimulation, controlling inflammation and limiting autoreactivity. Multiple transcript variants exist.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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