Thermo Fisher Scientific CD85g (ILT7) Monoclonal Antibody (eBio17G10.2 (17G10.2)), Functional Grade, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 3 publications 3 publications

Functional Assay (Functional)

Assay-Dependent

View 1 publication 1 publication

Product Specifications

Published species

Non-human primate

Host/Isotype

Mouse / IgG1

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio17G10.2 (17G10.2)

Conjugate

Functional Grade Functional Grade Functional Grade

View additional formats

• APC
• PE
• PerCP-Cyanine5.5
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2866016

Product Specific Information

Description: The eBio17G10.2 monoclonal antibody reacts with human immunoglobulin-like transcript 7 (ILT7), also known as CD85g. ILT7 is part of the leukocyte immunoglobulin-like receptor (LIR) gene family and associates with Fc epsilon R1 gamma to form a functional complex which transduces ITAM-mediated signals that negatively modulates TLR-induced type I IFN production by human plasmacytoid dendritic cells. ILT7 is expressed exclusively on plasmacytoid dendritic cells and is absent in monocyte derived DCs. Treatment with IL-3 or CpG causes a decrease in expression.

Double staining of human PBMCs with eBio17G10.2 and anti-pDC marker BDCA2 yields virtually complete co-staining. Cross linking of ILT7 with eBio17G10.2 antibody causes reduced transcription of IFN alpha1, IFN alpha2, IFN alpha4 and IFN beta.

Applications Reported: This eBio17G10.2 (17G10.2) antibody has been reported for use in flow cytometric analysis and functional assays.

Applications Tested: This eBio17G10.2 (17G10.2) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

ILT7 is a pDC-specific IgG-like transcript and is a member of the leukocyte immunoglobulin-like receptor gene family. This receptor, in complex with FcER1a, transduces ITAM-mediated signals which negatively modulate type I IFN production by pDC. ILT7 is thus a potential target to regulate Type 1 IFN production by pDC, which play a major role during viral infection, auto-immune diseases and anti-cancer immunity. Additionally, ILT7, along with CD123 and BDCA-2, can be used to identify or classify subsets of pDCalong with TLR expression and TLR mediated immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.