Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), Brilliant Ultra Violet 805, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

Unconjugated, Alexa Fluor 532, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV 395, Brilliant UV 496, Brilliant UV 563, Brilliant UV 615, Brilliant UV 661, Brilliant UV 737, Brilliant Violet 421, Brilliant Violet 605, Brilliant Violet 650, Brilliant Violet 711, Brilliant Violet 786, eFluor 450, eFluor 506, FITC, NovaFluor Blue 510, NovaFluor Blue 555, NovaFluor Blue 585, NovaFluor Blue 610-70S, NovaFluor Blue 660-120S, PE, PE-Cyanine7, PE-eFluor 610, PerCP-Cyanine5.5, Super Bright 436, Super Bright 600, Super Bright 645, Super Bright 780, 또는 Request custom conjugation

Product Specific Information

The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains such as C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A, and AKR. CD45.2 is expressed by all leukocytes in these strains.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes at ≤1.0 µg/test (100 µL volume, 10⁵–10⁸ cells/test). Optimize dilution for specific assays.
• Fluorochrome: Brilliant Ultra Violet™ 805 (BUV805), emission at 797 nm, suitable for 355 nm UV laser.
• Light Sensitivity: Protect from light to prevent photo-induced oxidation.
• Fixation: Compatible with IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark.
• Polymer Dye Panels: Use Super Bright Complete Staining Buffer (Product #SB-4401) or Brilliant Stain Buffer (Product #00-4409-75) to minimize non-specific interactions.
• Methanol Compatibility: Short-term methanol fixation is acceptable; avoid long-term methanol exposure (>1 hour).
• Excitation/Emission: 355 nm / 797 nm; Laser: Ultraviolet.

Trademark Notice: BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells and constitutes about 10% of lymphocyte surface proteins. It plays a key role in lymphocyte development and antigen signaling by regulating Src-family kinases. CD45.2 exists in multiple isoforms due to alternative splicing, differing in extracellular domains. It also participates in integrin-mediated signaling, cell survival, and apoptosis regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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