Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), Brilliant Ultra Violet 805, eBioscience

Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), Brilliant Ultra Violet 805

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test
Publications: View 1 publication

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 737
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue, Red, UV, Violet, Yellow series
• PE, PE-Cyanine5, 5.5, 7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45 (Leukocyte Common Antigen, LCA).
CD45 is expressed by all hematopoietic cells except mature erythrocytes and platelets. It plays a key role in lymphocyte activation through its tyrosine phosphatase activity.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse bone marrow cells at ≤0.5 µg/test (100 µL final volume). Optimize concentration per assay.

Brilliant Ultra Violet™ 805 (BUV805):

• Tandem dye emitting at 797 nm
• Compatible with UV (355 nm) laser-equipped cytometers
• Sensitive to photo-induced oxidation — protect from light

Staining Recommendations:

• Use Super Bright Complete Staining Buffer (SB-4401) or Brilliant Stain Buffer (00-4409-75) to minimize non-specific polymer interactions.

Fixation Compatibility:

• Samples can be stored in IC Fixation Buffer (00-8222) or Fix/Lyse Solution (00-5333) for up to 3 days at 4°C in the dark.
• Compatible with short-term methanol-based fixation (≤1 hour).

Excitation: 355 nm
Emission: 797 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD45 (Leukocyte Common Antigen, LCA) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells.
It constitutes ~10% of lymphocyte surface proteins and regulates Src-family kinases, antigen signaling, and cytokine receptor pathways.
CD45 isoforms differ by alternative splicing and are essential for T- and B-cell receptor signaling, apoptosis regulation, and cell survival.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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