Thermo Fisher Scientific Bcl-2 Monoclonal Antibody (8E12)
BCL-2 단백질을 검출하는 Mouse IgG1 단일클론 항체로, IHC, ICC/IF, Flow Cytometry, ELISA 등 다양한 응용에 적합. Human 및 Mouse 시료에서 반응하며, 세포 사멸 관련 연구에 유용. 장기 보관 시 -20°C에서 보관 권장.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | - |
| Flow Cytometry (Flow) | 1:200–1:400 | - |
| ELISA | 1:10,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8E12 |
| Immunogen | Synthetic peptide corresponding to residues surrounding BCL-2, conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | Ascites |
| Contains | 0.03% Sodium Azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet Ice |
| RRID | AB_10985538 |
Product Specific Information
MA5-15668 targets BCL-2 in indirect ELISA, FACS, IF, and IHC applications and shows reactivity with Human and Mouse samples.
The MA5-15668 immunogen is a synthetic peptide corresponding to residues surrounding BCL-2, conjugated to KLH.
MA5-15668 detects BCL-2, which has a predicted molecular weight of approximately 26 kDa.
Target Information
BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are characterized by their ability to dimerize to further modulate apoptosis.
Bag1 forms a heterodimer with BCL-2, enhancing its anti-apoptotic effect. Bax and Bak play critical roles in cytochrome c release from mitochondria, initiating apoptosis.
Constitutive expression of BCL-2, such as in translocation to the Ig heavy chain locus, is associated with follicular lymphoma.
In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas normal or hyperplastic germinal centers are negative.
Two transcript variants of BCL-2, produced by alternate splicing, differ at their C-terminal ends. Overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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