Thermo Fisher Scientific SMAD3 Monoclonal Antibody (5G11)
SMAD3 단백질 검출용 Mouse Monoclonal Antibody (Clone 5G11). Western blot, ICC/IF, ELISA 등 다양한 응용 가능. 인간 및 생쥐 시료 반응성. 비접합형 액상 항체로 장기 보관 시 -20°C 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 3 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | View 1 publication |
| ELISA | 1:10,000 | View 1 publication |
| in situ PLA (PLA) | – | View 1 publication |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5G11 |
| Immunogen | Purified recombinant fragment of human SMAD3 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | Ascites |
| Contains | 0.03% Sodium Azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet Ice |
| RRID | AB_10983274 |
Product Specific Information
MA5-15663 targets SMAD3 in indirect ELISA, ICC, IF, and WB applications, showing reactivity with human and mouse samples.
The immunogen is a purified recombinant fragment of human SMAD3 expressed in E. coli.
MA5-15663 detects SMAD3, which has a predicted molecular weight of approximately 48 kDa.
Target Information
SMAD3 belongs to the SMAD family of proteins, similar to the gene products of the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma.
SMAD proteins act as signal transducers and transcriptional modulators mediating multiple signaling pathways.
SMAD3 functions as a transcriptional modulator activated by transforming growth factor-beta (TGF-β) and is implicated in the regulation of carcinogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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