Thermo Fisher Scientific CD39 Monoclonal Antibody (eBioA1 (A1)), NovaFluor Blue 660-120S, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.8 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• APC-eFluor 780
• Brilliant UV 805
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Custom conjugation available

Product Specific Information

Description:
The eBioA1 monoclonal antibody reacts with human CD39 (ENTPD1 or NTPDase), an integral membrane protein with two transmembrane domains that exists as a homotetramer. CD39 functions to remove toxic extracellular ATP by converting it to ADP or AMP and works with CD73 to hydrolyze ATP. Expression is found on subsets of T cells, B cells, dendritic cells, and weakly on monocytes and granulocytes. CD39 and CD73 are also expressed on regulatory T cells (Treg), aiding their migration into inflamed tissues.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported:

• Flow cytometric analysis

Applications Tested:

• Pre-titrated and tested on normal human peripheral blood cells
• Recommended use: 5 µL (0.8 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)
• Optimize titration for each assay

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
  • Add 5 µL per sample to antibody cocktails before combining with cells.
  • For single-color controls: 5 µL per 100 µL cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures designed to minimize spillover and spread.

Optical Properties:

• Excitation: 509 nm
• Emission: 665 nm
• Laser: 488 nm (Blue)

Target Information

CD39 is a 70–100 kDa cell surface antigen expressed on peripheral blood B cells, monocytes, T cell clones, and weakly on granulocytes. It exhibits intrinsic ecto-ATPase activity and is upregulated on T and B cells upon activation.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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