Thermo Fisher Scientific AHA1 Monoclonal Antibody (25F2.D9), FITC
AHA1 단백질을 인식하는 FITC 결합 단클론 항체로, WB, IHC, ICC, ELISA, IP 등에 사용 가능. 인간, 마우스, 랫트 반응성. 단백질 G로 정제된 액상 형태로, 4°C 암소 보관. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:1,000 |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | 1:1,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 25F2.D9 |
| Immunogen | Recombinant Full Length Mouse Aha1 Protein |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1.2 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.3, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931688 |
Available Formats
- Unconjugated (MA5-27641)
- APC (MA5-45233)
- PE (MA5-45236)
- PerCP (MA5-45235)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45234 was sufficient for detection of Aha1 in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody. Detects approximately 38 kDa, may run up to 45 kDa on SDS-PAGE.
Target Information
Acts as a co-chaperone of HSP90AA1. Activates the ATPase activity of HSP90AA1, increasing its chaperone activity. Competes with inhibitory co-chaperones FNIP1 and TSC1 for binding to HSP90AA1, providing a reciprocal regulatory mechanism for client protein chaperoning. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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