Thermo Fisher Scientific RAB11B Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (IHC)

-

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Immunocytochemistry (ICC/IF)

1:50-1:200

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human, Non-human primate

Published species

Human, Sea urchin

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues M(1) G T R D D E Y D Y L F K V V L I C(17) of human Rab11. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RAB11B,uniProtId:Q15907-1,ncbiNodeId:9606,antigenRange:1-17,antigenLength:218,antigenImageFileName:PA1-775_RAB11B_Q15907-1_Rabbit.svg,antigenImageFileNamePDP:PA1-775_RAB11B_Q15907-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2173598

Product Specific Information

PA1-775 detects Rab 11 from human and non-human primate samples. This antibody detects both A and B isoforms.

PA1-775 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~22 kDa protein representing Rab 11 in CV1, PC3, and HeLa cell extract. Another less prominent, unknown band is also detected by this antibody at ~35 kDa.

The PA1-775 immunizing peptide corresponds to amino acid residues 1-17 from human Rab 11. This sequence is completely conserved in human, mouse and rat and 94% conserved in C. elegans and amphibians. This peptide (Cat. # PEP-195) is available for use in neutralization and control experiments.

Target Information

Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 Rab proteins that have been described in mammals. Rab 11 is a known marker for protein trafficking, sorting, and recycling in the endosomal pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.