Thermo Fisher Scientific Phospho-Synaptotagmin 1/2 (Thr202, Thr199) Polyclonal Antibody
Phospho-Synaptotagmin 1/2 (Thr202, Thr199) 단백질의 인산화 형태를 검출하는 rabbit polyclonal 항체. Western blot과 IHC(P)에서 사용 가능하며, 인간, 마우스, 랫트에 반응. 고순도 항원 친화 크로마토그래피 정제 제품으로 신경전달 관련 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human Synaptotagmin 1 around the phosphorylation site of Threonine 202 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2553665 |
Product Specific Information
- Detects endogenous protein at approximately 60 kDa.
- Purity >95% confirmed by SDS-PAGE.
Target Information
Synaptotagmins are a large gene family of synaptic vesicle type III integral membrane proteins that regulate exocytosis and endocytosis, playing key roles in neurotransmitter secretion.
Calcium binding to Synaptotagmin I triggers neurotransmitter release at the synapse. Synaptotagmin II is phosphorylated by WNK1 to regulate calcium-dependent interactions. Synaptotagmin III participates in calcium-dependent exocytosis in endocrine cells and neurons, while Synaptotagmin IV is highly expressed in neuronal tissues, especially in the hippocampus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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