Thermo Fisher Scientific Phospho-SHP-1 (Tyr536) Polyclonal Antibody
SHP-1 단백질의 Tyr536 인산화 부위를 인식하는 Rabbit Polyclonal 항체. Western blot 및 IHC(P) 분석에 적합하며, Human, Mouse, Rat에 반응. 항원 친화 크로마토그래피로 정제된 고순도 항체로, PBS/glycerol buffer에 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human SHP-1 around the phosphorylation site of Tyrosine 536 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C for short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553660 |
Product Specific Information
- Detects endogenous SHP-1 protein at a molecular weight of approximately 68 kDa.
- Purity is greater than 95% by SDS-PAGE.
Target Information
SHP-1 (PTPN6) is a non-receptor protein tyrosine phosphatase expressed primarily in hematopoietic cells. It consists of two SH2 domains, a tyrosine phosphatase catalytic domain, and a carboxy-terminal regulatory domain. SHP-1 functions to remove phosphates from target proteins, thereby downregulating several tyrosine kinase-regulated signaling pathways.
In hematopoietic cells, SHP-1 binds to tyrosine-phosphorylated erythropoietin receptors (EpoR) to negatively regulate hematopoietic growth. Overexpression in epithelial cells leads to dephosphorylation of the Ros receptor tyrosine kinase and suppression of Ros-dependent proliferation. In myeloid cells, SHP-1 associates with the IL-3R beta chain to downregulate IL-3-induced phosphorylation and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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