Thermo Fisher Scientific V5 Tag Monoclonal Antibody (2F11F7), Alexa Fluor 555
V5 태그 단백질 검출용 마우스 단클론 항체로 Alexa Fluor 555 형광으로 표지됨. Western blot, ICC/IF, IP에 적합. 단백질 A로 정제된 고순도 항체로 4°C 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2F11F7 |
| Immunogen | V5 synthetic peptide: Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr |
| Conjugate | Alexa Fluor™ 555 |
| Excitation / Emission Max | 553 / 568 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2610631 |
Additional Formats
Target Information
Epitope tags provide a method to localize gene products in various cell types, study protein topology and complexes, identify associated proteins, and characterize newly identified or low-abundance proteins when protein-specific antibodies are unavailable.
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5).
The V5 tag is typically used with all 14 amino acids (GKPIPNPLLGLDST), though shorter 9-amino-acid sequences (IPNPLLGLD) are also used.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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