Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), Brilliant Ultra Violet 661, eBioscience

Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), Brilliant Ultra Violet 661, eBioscience

Applications

• Flow Cytometry (Flow): Tested dilution 0.5 µg/test
  Publications: -

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 563
• Brilliant UV 615
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 786
• eFluor 450
• eFluor 506
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa Ig superfamily member involved in peripheral tolerance and autoimmune disease regulation. PD-1 is expressed mainly on activated T and B lymphocytes and interacts with PD-L1 (B7-H1) and PD-L2 (B7-DC).
J43 inhibits PD-L1-Ig and PD-L2-Ig binding to PD-1/BHK cells and modulates immune responses in vivo.
This antibody is validated for flow cytometric analysis of mouse splenocytes and can be used at ≤0.5 µg/test (100 µL final volume). Optimize cell number empirically (10⁵–10⁸ cells/test).

Fluorochrome Information:
Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm and is compatible with UV (355 nm) laser-equipped cytometers. Protect from light due to photo-induced oxidation sensitivity.

Staining Buffer Recommendation:
Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific polymer interactions when using multiple Brilliant dyes.

Fixation:
Samples can be stored up to 3 days at 4°C in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) with minimal fluorescence loss.

Excitation/Emission:

• Excitation: 350 nm
• Emission: 660 nm
• Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

T-cell activation is regulated by PD-1 (PDCD-1), which binds ligands PD-L1 and PD-L2 to deliver inhibitory signals from antigen-presenting cells, maintaining immune tolerance. PD-1 deficiency leads to hyperactive immune responses and autoimmune phenotypes in mice.
Although PD-1 has a predicted molecular weight of 55 kDa, it often migrates at a higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지