Thermo Fisher Scientific SIRT1 Recombinant Rabbit Monoclonal Antibody (SZ04-01)
SIRT1 단백질을 표적하는 Thermo Fisher의 재조합 토끼 단클론 항체. Western blot, IHC, ICC/IF에 적합하며 높은 특이성과 재현성을 제공. Protein A 정제, 액상 형태로 제공되며 장기 보관 시 -20°C 권장. 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SZ04-01 |
| Immunogen | Synthetic peptide within Human SIRT1 (aa 698–747) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809324 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, and individual clones are screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
NAD-dependent protein deacetylase sirtuin-1 (SIRT1) links transcriptional regulation to intracellular energetics and coordinates cellular functions such as cell cycle control, DNA damage response, metabolism, apoptosis, and autophagy.
SIRT1 modulates chromatin function through histone deacetylation and influences histone/DNA methylation, leading to transcriptional repression.
It is essential in skeletal muscle cell differentiation and mediates inhibitory effects on myoblast differentiation under low nutrient conditions via AMPK and NAMPT pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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