Thermo Fisher Scientific CD203c Monoclonal Antibody (NP4D6), PE-Cyanine7, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.125 µg)/test

Product Specifications

Additional Formats

• APC
• PE
• Request custom conjugation

Product Specific Information

This NP4D6 monoclonal antibody reacts with human CD203c (E-NPP3), a type II transmembrane protein expressed on basophils and mast cells. CD203c expression increases upon basophil activation by allergen or IgE crosslinking, serving as an indicator of IgE-mediated allergic responses.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated for flow cytometry on normal human peripheral blood cells
  • Recommended use: 5 µL (0.125 µg)/test (100 µL final volume, 10⁵–10⁸ cells/test)
• Light Sensitivity: Protect from light due to photo-induced oxidation
• Fixation:
  • IC Fixation Buffer (Product #00-8222-49)
  • 1-step Fix/Lyse Solution (Product #00-5333-54)
  • Samples stable up to 3 days at 4°C in dark
• Excitation/Emission: 488–561 nm / 775 nm
• Laser Compatibility: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD203c (E-NPP3) encodes an ectoenzyme involved in hydrolysis of extracellular nucleotides. These enzymes exhibit ATPase and ATP pyrophosphatase activities and are type II transmembrane proteins. Expression has been observed in glioma cells, prostate, uterus, pancreas, and basophils.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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