Thermo Fisher Scientific CD203c Monoclonal Antibody (NP4D6), PE-Cyanine7, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

-

Product Specifications

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

NP4D6

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

View additional formats

• APC
• PE
• Request custom conjugation

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_2573423

Product Specific Information

Description: This NP4D6 monoclonal antibody reacts with human CD203c, which is also known as ectonucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP3). ENPP family members catalyze the hydrolysis of pyrophosphate and phosphodiester bonds to nucleoside 5-monophosphates. A type II transmembrane protein, CD203c is expressed on basophils and mast cells. CD203c expression is upregulated on basophils activated by allergen or IgE crosslinking. Therefore, CD203c is used as an indicator of basophil activation in various IgE-mediated allergic responses.

Applications Reported: This NP4D6 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This NP4D6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The protein encoded by this gene belongs to a series of ectoenzymes that are involved in hydrolysis of extracellular nucleotides. These ectoenzymes possess ATPase and ATP pyrophosphatase activities and are type II transmembrane proteins. Expression of the related rat mRNA has been found in a subset of immature glial cells and in the alimentary tract. The corresponding rat protein has been detected in the pancreas, small intestine, colon, and liver. The human mRNA is expressed in glioma cells, prostate, and uterus. Expression of the human protein has been detected in uterus, basophils, and mast cells. [provided by RefSeq, Jul 2008]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.