Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (3D12), PE-Cyanine7, eBioscience
인간 CCR7(CD197)을 인식하는 3D12 클론의 Rat IgG2a 단일클론 항체로, PE-Cyanine7 형광체가 결합되어 있습니다. Flow Cytometry에 최적화되어 있으며, 5 µL/test로 사용 가능합니다. T세포 및 수지상세포의 분화와 이동 연구에 유용합니다.
- 카탈로그번호
- 25-1979-4x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (3D12), PE-Cyanine7, eBioscience
Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (3D12), PE-Cyanine7, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.25 µg)/test
View 17 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3D12 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573421 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 563, 661, 737
- Brilliant Violet 421, 480, 711, 786
- FITC
- NovaFluor Red 685, 700, 755
- NovaFluor Yellow 700
- PE
- PE-Cyanine5.5
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600, 645, 780
- Request custom conjugation
Product Specific Information
The 3D12 monoclonal antibody reacts with human CCR7 (CD197), also known as EBI-1. CCR7 is a G-protein-coupled chemokine receptor with seven transmembrane domains, functioning as a receptor for 6Ckine/SLC (CCL19, CCL21). It is expressed on T cells and dendritic cells (DCs), playing roles in migration, chemotaxis, and maturation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated and validated for human peripheral blood cells at 5 µL (0.25 µg)/test
- Recommended Incubation: ≥45 minutes at 2–8°C
- Light Sensitivity: Protect from light (photo-induced oxidation)
- Fixation: Compatible with IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333)
- Excitation/Emission: 488–561 nm / 775 nm
- Laser Compatibility: Blue, Green, Yellow-Green lasers
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CCR7 is a member of the G protein-coupled receptor family and is induced by Epstein Barr virus (EBV). It is expressed in blood, bone marrow, lymph node, and intestine, particularly in lymphoid tissues and activated B/T lymphocytes. CCR7 controls migration of memory T cells to inflamed tissues and stimulates dendritic cell maturation. Its ligands include CCL19 and CCL21.
Receptors in the C-C chemokine family include CCR1–CCR5 and the Duffy antigen, all critical for T cell chemotaxis and phagocyte migration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.