Thermo Fisher Scientific CD199 (CCR9) Monoclonal Antibody (eBioCW-1.2 (CW-1.2)), PE-Cyanine7, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test
• Publications: View 4 publications

Product Specifications

Available Formats

• APC
• eFluor 450
• FITC
• PE
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
The eBioCW-1.2 monoclonal antibody reacts with mouse CCR9 (CD199), the receptor for thymus-expressed chemokine (TECK). CCR9 is a G protein-coupled receptor involved in the trafficking of T cell progenitors within the thymus. Expression begins at the DN3 stage, peaks at the DP stage, and decreases in CD4+ or CD8+ SP thymocytes. CCR9-deficient mice show mild impairment in thymocyte development. In peripheral tissues, CCR9 is expressed on naïve CD8+ T cells but not on naïve CD4+ T cells.

Applications Reported:
Suitable for flow cytometric analysis.

Applications Tested:
Tested on mouse thymocytes. Recommended ≤0.5 µg/test (in 100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimize titration for best performance.

Light Sensitivity:
Tandem dye is photo-sensitive. Protect from light.

Fixation:
Samples may be stored in:

• IC Fixation Buffer (Product # 00-8222-49)
• 1-step Fix/Lyse Solution (Product # 00-5333-54)
  Store up to 3 days at 4°C in the dark. Clone-specific performance should be empirically determined.

Excitation/Emission:
488–561 nm / 775 nm
Compatible Lasers: Blue, Green, Yellow-Green

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

The CCR9 gene encodes a beta chemokine receptor, a seven-transmembrane G protein-coupled receptor. Chemokines and their receptors regulate thymocyte migration and maturation under normal and inflammatory conditions. The ligand for CCR9 is CCL25. Differential expression in T lymphocytes of the small intestine and colon suggests a role in thymocyte recruitment and immune specialization in the gastrointestinal tract. The gene is located in the chemokine receptor cluster region and has two alternatively spliced transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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