Thermo Fisher Scientific HCN1 Monoclonal Antibody (S70)
HCN1 단백질을 인식하는 S70 클론의 마우스 모노클로날 항체로, WB, IHC, IP, AM 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. 단백질 G 정제, PBS/glycerol buffer, -20°C 보관. 연구용으로만 사용 가능.
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Thermo Fisher Scientific HCN1 Monoclonal Antibody (S70)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunoprecipitation (IP) | Assay-dependent |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S70 |
| Immunogen | Fusion protein amino acids 778–910 (C terminus) of rat HCN1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735210 |
Available Conjugated Formats
Product Specific Information
1 µg/mL of MA5-27589 was sufficient for detection of HCN1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 100 kDa. No cross-reactivity against HCN2.
Target Information
HCN1 encodes hyperpolarization-activated cation channels of the HCN gene family, contributing to spontaneous rhythmic activity in both heart and brain.
HCN1 and HCN2 are pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides.
HCN1 plays a key role in motor learning and neuronal integration by cerebellar Purkinje cells and shapes autonomous neuronal activity and network oscillations.
Expression of HCN1 is enriched in the cerebral cortex, hippocampus, cerebellum, and facial motor nucleus.
HCN2 is abundant in mamillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body.
These regional variations may influence neuronal pacemaker activity across brain systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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