Thermo Fisher Scientific TNFR1 (soluble) Polyclonal Antibody, Biotin, PeproTech
Human TNFR1(sTNFR1)을 인식하는 Biotin 결합 Rabbit Polyclonal Antibody로, ELISA 및 Western blot에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, -20°C에서 보관합니다. 인간 TNFR1 단백질 검출 및 신호전달 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | – |
| Flow Cytometry (Flow) | – | 1 publication |
| ELISA | 0.25–1.0 µg/mL | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived, 18.3 kDa Recombinant Human TNF Receptor Type I |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
Product Specific Information
AA Sequence of recombinant protein:
MDSVCPQGKY IHPQNNSICC TKCHKGTYLY NDCPGPGQDT DCRECESGSF TASENHLRHC LSCSKCRKEM GQVEISSCTV DRDTVCGCRK NQYRHYWSEN LFQCFNCSLC LNGTVHLSCQ EKQNTVCTCH AGFFLRENEC VSCSNCKKSL ECTKLCLPQI EN.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sTNF Receptor Type I. Anti-Human sTNF Receptor Type I-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect Human sTNF Receptor Type I by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sTNF Receptor Type I (500-P143) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human sTNF Receptor Type I.
Western Blot:
To detect hsTNF-Receptor I by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hsTNF-Receptor I is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Package Information:
500-P143BT-1MG will be provided as 2 × 500 µg.
Target Information
TNFR1 (Tumor necrosis factor receptor 1) belongs to the tumor necrosis factor superfamily and is one of the major TNF-alpha receptors. TNFR1 plays an important role in cytokine-mediated signaling, positive regulation of the NF-κB pathway, angiogenesis, and gene expression regulation.
The extracellular domain of TNFR1 is also released into the circulatory system as soluble TNFR1 (sTNFR1). In humans, the TNFR1 gene is located on chromosome 12.
Proteins such as BAG4/SODD, TRADD, and TRAF2 interact with TNFR1 and regulate its signal transduction. Germline mutations in the extracellular domain of TNFR1 are associated with autosomal dominant periodic fever syndrome, with impaired receptor clearance being a possible mechanism.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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