Thermo Fisher Scientific RANK (soluble) Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MQIAPPCTSE KHYEHLGRCC NKCEPGKYMS SKCTTTSDSV CLPCGPDEYL DSWNEEDKCL LHKVCDTGKA LVAVVAGNST TPRRCACTAG YHWSQDCECC RRNTECAPGL GAQHPLQLNK DTVCKPCLAG YFSDAFSSTD KCRPWTNCTF LGKRVEHHGT EKSDAVCSSS LPARK.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sRANK Receptor. Anti-Human sRANK Receptor-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human sRANK Receptor by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sRANK Receptor (500-P144) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human sRANK Receptor.

Western Blot:
To detect hsRANK Receptor by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
Used with compatible secondary reagents, the detection limit for Recombinant hsRANK Receptor is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P144BT-1MG is provided as 2 × 500 µg.

Target Information

RANK is a member of the tumor necrosis factor receptor (TNFR) family. The full-length mouse RANK cDNA encodes a type I transmembrane protein with extracellular and cytoplasmic domains.
The extracellular domain contains two potential N-linked glycosylation sites.
RANK shares significant amino acid homology with other members of the TNFR family in its extracellular four cysteine-rich repeats.
Human and murine RANK share 81% amino acid identity in their extracellular domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.