Thermo Fisher Scientific CXCL6 Polyclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Immunostaining (IS)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse, Rat

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Murine LIX (CXCL6) (92 a.a.)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929477

Product Specific Information

AA Sequence of recombinant protein: APSSVIAATE LRCVCLTVTP KINPKLIANL EVIPAGPQCP TVEVIAKLKN QKEVCLDPEA PVIKKIIQKI LGSDKKKAKR NALAVERTAS VQ.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Mutine LIX (CXCL6). Anti-Murine LIX (CXCL6)-specific antibody was purified by affinity chromatography employing an immobilized Murine LIX (CXCL6) matrix.

Sandwich ELISA: To detect Murine LIX (CXCL6) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine LIX (CXCL6) (500-P146Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine LIX (CXCL6).

Western Blot: To detect Murine LIX (CXCL6) by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine LIX (CXCL6) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

CXCL6 is a chemotactic for neutrophil granulocytes. It signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.