Thermo Fisher Scientific RFP Pre-adsorbed Polyclonal Antibody, Biotin
RFP 및 변형 단백질(mCherry, tdTomato 등)에 특이적인 Biotin 결합 폴리클로날 항체. Western blot, IHC, ELISA, IP 등 다양한 응용에 적합. 높은 특이성과 재현성을 가진 Rabbit IgG 기반 항체로 연구용에 최적화됨.
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Thermo Fisher Scientific RFP Pre-adsorbed Polyclonal Antibody, Biotin
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:10,000–1:80,000 |
| Immunoprecipitation (IP) | 1:2,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Red Fluorescent Protein (RFP) fusion protein, full-length (234 aa) from Discosoma coral |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid; dilute only prior to use.
Reactivity:
This antibody reacts with RFP and variants including mCherry, tdTomato, mBanana, mOrange, mPlum, and mStrawberry.
Immunoelectrophoresis shows a single precipitin arc against anti-biotin, anti-rabbit serum, and purified RFP (Discosoma).
No cross-reactivity observed with human, mouse, or rat serum proteins.
ELISA confirmed specificity with <0.1% non-target signal.
Target Information
Red Fluorescent Protein (RFP) is derived from Discosoma sp. (Uniprot ID: Q9U6Y8).
It is widely used as a molecular tag for studying:
- Protein localization
- Reporter gene expression
- Protein–protein interactions
RFP naturally forms a homotetramer, but monomeric variants are available for diverse applications.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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